pEGFP-h53BP1 (siRNA resistant)
(Plasmid #110301)

• Purpose: Mammalian expression of a EGFP-tagged full length human 53BP1 (resistant to siRNA targeting AGAACGAGGAGACGGTAATAGTGGG)
• Depositing Lab: Chris Kok-Lung Chan
• Publication: Tiwari et al Nat Commun. 2018 Feb 14;9(1):677. doi: 10.1038/s41467-018-03098-y.

Backbone

• Vector backbone: pEGFP
• Backbone manufacturer: Clontech
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: p53-binding protein 1
• Species: H. sapiens (human)
• Mutation: GAACGAGGA to GAGCGGGGC
• Entrez Gene: TP53BP1 (a.k.a. 53BP1, TDRD30, p202, p53BP1)
• Tag / Fusion Protein:
  • EGFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)
• 3′ cloning site: unknown (unknown if destroyed)
• 5′ sequencing primer: unknown

Resource Information

• Supplemental Documents:
  • pEGFP-C3-h53BP1(siRNA-Res) (1).gb
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

resistant to siRNA targeting AGAACGAGGAGACGGTAATAGTGGG