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pSV272-PfMBP-HypDAE
(Plasmid #110296)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 110296 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSV-PfMBP
  • Backbone manufacturer
    Stubbe Lab (see DOI:10.1021/acs.biochem.5b01092)
  • Backbone size w/o insert (bp) 6575
  • Total vector size (bp) 7484
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    t-4-Hydroxy-L-proline Dehydratase Activating Enzyme
  • Alt name
    HypD-AE
  • Alt name
    t4LHypD-AE
  • Species
    Clostridium difficile 70-100-2010
  • Insert Size (bp)
    909
  • GenBank ID
    WP_102822675.1
  • Promoter T7
  • Tags / Fusion Proteins
    • His6 (N terminal on backbone)
    • Maltose Binding Protein (N terminal on backbone)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer TAATACGACTCACTATAGGG
  • 3′ sequencing primer GCTAGTTATTGCTCAGCGG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSV272-PfMBP-HypDAE was a gift from Emily Balskus (Addgene plasmid # 110296 ; http://n2t.net/addgene:110296 ; RRID:Addgene_110296)

  • For your References section:

    A prominent glycyl radical enzyme in human gut microbiomes metabolizes trans-4-hydroxy-l-proline. Levin BJ, Huang YY, Peck SC, Wei Y, Martinez-Del Campo A, Marks JA, Franzosa EA, Huttenhower C, Balskus EP. Science. 2017 Feb 10;355(6325). pii: 355/6325/eaai8386. doi: 10.1126/science.aai8386. 10.1126/science.aai8386 PubMed 28183913
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