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p029 End-1::EnR
(Plasmid #11029)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 11029 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCS2
  • Backbone size w/o insert (bp) 4095
  • Vector type
    xenopus, mammalian, avian, and zebrafish

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    End1::EnR
  • Alt name
    END-1
  • Alt name
    Engrailed repressor
  • Species
    END-1 is from C. Elegans, EnR is from Drophila
  • Insert Size (bp)
    1300
  • Mutation
    Dominant inhibitory GATA-binding protein End1::EnR was created by fusing the C-terminal (aa106-221) of END-1 to the NH2-terminal repressor domain (aa2-296) of Drosophila Engrailed.
  • GenBank ID
    NM_074074
  • Entrez Gene
    end-1
  • Entrez Gene
    en (a.k.a. Dmel_CG9015, 153867_at, Apa, CG9015, Dmel\CG9015, EN, En, EnR, Eng, Engr, Engrailed, Engrailed/Invected, Es, V, en1, spa2, spt)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer SP6
  • 3′ sequencing primer T3 or M13 reverse
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p029 End-1::EnR was a gift from Ramesh Shivdasani (Addgene plasmid # 11029 ; http://n2t.net/addgene:11029 ; RRID:Addgene_11029)

  • For your References section:

    Action of the Caenorhabditis elegans GATA factor END-1 in Xenopus suggests that similar mechanisms initiate endoderm development in ecdysozoa and vertebrates. Shoichet SA, Malik TH, Rothman JH, Shivdasani RA. Proc Natl Acad Sci U S A. 2000 Apr 11. 97(8):4076-81. 10.1073/pnas.97.8.4076 PubMed 10760276
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