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Addgene

pATLIC
(Plasmid #110287)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 110287 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBAD
  • Backbone size w/o insert (bp) 3986
  • Total vector size (bp) 5519
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    yfaL
  • Alt name
    autotransporter
  • Species
    E. coli
  • Insert Size (bp)
    1533
  • GenBank ID
    AAA74094
  • Promoter araBAD promoter

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer GAAGGAGATATAAGGATGCGGATTATCTTTCTACGCAAG
  • 3′ sequencing primer ATGATGGTGATGGTGCCATTTCACCGTCATCGACAAA
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Addgene QC finds S92G, G99S, and K330R mutations in yfaL as well as a stop codon inserted before the His tag. The depositor notes these are not of functional concern

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pATLIC was a gift from In-Geol Choi (Addgene plasmid # 110287 ; http://n2t.net/addgene:110287 ; RRID:Addgene_110287)

  • For your References section:

    Functional cell surface display and controlled secretion of diverse Agarolytic enzymes by Escherichia coli with a novel ligation-independent cloning vector based on the autotransporter YfaL. Ko HJ, Park E, Song J, Yang TH, Lee HJ, Kim KH, Choi IG. Appl Environ Microbiol. 2012 May;78(9):3051-8. doi: 10.1128/AEM.07004-11. Epub 2012 Feb 17. 10.1128/AEM.07004-11 PubMed 22344647
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