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Addgene

ACTIN-Cxcl5-PGK-Cre
(Plasmid #110278)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 110278 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    GFP.Cre empty vector
  • Backbone size w/o insert (bp) 11000
  • Total vector size (bp) 11400
  • Modifications to backbone
    B-actin promoter replaces the Ubc promoter, Cxcl5 replaces GFP
  • Vector type
    Mammalian Expression, Lentiviral, Cre/Lox

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    chemokine (C-X-C motif) ligand 5
  • Alt name
    Cxcl5
  • Alt name
    ENA-78
  • Alt name
    GCP-2
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    399
  • Mutation
    Wild type
  • GenBank ID
    NM_009141.3
  • Entrez Gene
    Cxcl5 (a.k.a. AMCF-II, Cxcl6, ENA-78, GCP-2, LIX, Scyb5, Scyb6)
  • Promoter B-actin
  • Tag / Fusion Protein
    • No fusion genes

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoR1 (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer Unknown
  • 3′ sequencing primer MSCV-rev
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    cyclization recombinase
  • Alt name
    Cre
  • Species
    Enterobacteria phage P1
  • Insert Size (bp)
    1032
  • GenBank ID
    NC_005856.1
  • Promoter Pgk

Cloning Information for Gene/Insert 2

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    ACTIN-Cxcl5-PGK-Cre was a gift from Trudy Oliver (Addgene plasmid # 110278 ; http://n2t.net/addgene:110278 ; RRID:Addgene_110278)

  • For your References section:

    The Lineage-Defining Transcription Factors SOX2 and NKX2-1 Determine Lung Cancer Cell Fate and Shape the Tumor Immune Microenvironment. Mollaoglu G, Jones A, Wait SJ, Mukhopadhyay A, Jeong S, Arya R, Camolotto SA, Mosbruger TL, Stubben CJ, Conley CJ, Bhutkar A, Vahrenkamp JM, Berrett KC, Cessna MH, Lane TE, Witt BL, Salama ME, Gertz J, Jones KB, Snyder EL, Oliver TG. Immunity. 2018 Oct 16;49(4):764-779.e9. doi: 10.1016/j.immuni.2018.09.020. 10.1016/j.immuni.2018.09.020 PubMed 30332632
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