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Addgene

pCDB367
(Plasmid #110277)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 110277 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCDB364
  • Backbone size w/o insert (bp) 5919
  • Total vector size (bp) 6044
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    gHEEE_02
  • Species
    Synthetic
  • Insert Size (bp)
    129
  • Promoter T7
  • Tags / Fusion Proteins
    • 10-His (N terminal on backbone)
    • mature DsbC (i.e. no signal peptide) (N terminal on backbone)
    • Tev recognition peptide (N terminal on backbone)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer AGCAATGGCACACTTG
  • 3′ sequencing primer GCTAGTTATTGCTCAGCGG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCDB367 was a gift from Christopher Bahl (Addgene plasmid # 110277 ; http://n2t.net/addgene:110277 ; RRID:Addgene_110277)

  • For your References section:

    Cytosolic expression, solution structures, and molecular dynamics simulation of genetically encodable disulfide-rich de novo designed peptides. Buchko GW, Pulavarti SVSRK, Ovchinnikov V, Shaw EA, Rettie SA, Myler PJ, Karplus M, Szyperski T, Baker D, Bahl CD. Protein Sci. 2018 Aug 26. doi: 10.1002/pro.3453. 10.1002/pro.3453 PubMed 30152054
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