pCDB368
(Plasmid
#110276)
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PurposegHH_44 with a N-terminal 10-His DsbC TEV-peptide fusion
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 110276 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCDB364
- Backbone size w/o insert (bp) 5919
- Total vector size (bp) 5999
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namegHH_44
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SpeciesSynthetic
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Insert Size (bp)84
- Promoter T7
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Tags
/ Fusion Proteins
- 10-His (N terminal on backbone)
- mature DsbC (i.e. no signal peptide) (N terminal on backbone)
- Tev recognition peptide (N terminal on backbone)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer AGCAATGGCACACTTG
- 3′ sequencing primer GCTAGTTATTGCTCAGCGG (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCDB368 was a gift from Christopher Bahl (Addgene plasmid # 110276 ; http://n2t.net/addgene:110276 ; RRID:Addgene_110276) -
For your References section:
Cytosolic expression, solution structures, and molecular dynamics simulation of genetically encodable disulfide-rich de novo designed peptides. Buchko GW, Pulavarti SVSRK, Ovchinnikov V, Shaw EA, Rettie SA, Myler PJ, Karplus M, Szyperski T, Baker D, Bahl CD. Protein Sci. 2018 Aug 26. doi: 10.1002/pro.3453. 10.1002/pro.3453 PubMed 30152054
