pCDB364
(Plasmid #110275)

• Purpose: (Empty Backbone) N-terminal 10-His DsbC TEV-peptide fusion
• Depositing Lab: Christopher Bahl
• Publication: Buchko et al Protein Sci. 2018 Aug 26. doi: 10.1002/pro.3453.

Backbone

• Vector backbone: pCDB364
• Backbone size (bp): 5919
• Modifications to backbone: started with pET29 and exchanged the new multiple cloning site
• Vector type: Bacterial Expression
• Promoter: T7
• Tags / Fusion Proteins:
  • 10-His (N terminal on backbone)
  • mature DsbC (i.e. no signal peptide) (N terminal on backbone)
  • TEV cleavage peptide (N terminal on backbone)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: AGCAATGGCACACTTG
• 3′ sequencing primer: GCTAGTTATTGCTCAGCGG

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

built from pET29b