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Addgene

MTHFR (MTHFRA-c042)
(Plasmid #110271)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 110271 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFB-CT10HF-LIC
  • Backbone manufacturer
    Nicola Burgess-Brown (Addgene plasmid # 39191)
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MTHFR
  • Alt name
    methylenetetrahydrofolate reductase
  • Species
    H. sapiens (human)
  • Entrez Gene
    MTHFR
  • Promoter polyhedrin promoter
  • Tag / Fusion Protein
    • TEV-His10-Flag (C terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer polyhedrin forward (5'-AAATGATAACCATCTCGC-3')
  • 3′ sequencing primer SV40pA-R
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Use SF9 cells for expression. PDB: 6FCX

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MTHFR (MTHFRA-c042) was a gift from Nicola Burgess-Brown (Addgene plasmid # 110271 ; http://n2t.net/addgene:110271 ; RRID:Addgene_110271)
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