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IQSEC2 (IQSEC2A-c001)
(Plasmid #110259)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 110259 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pNIC28-10His
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    IQSEC2
  • Alt name
    IQ motif and Sec7 domain 2
  • Species
    H. sapiens (human)
  • Entrez Gene
    IQSEC2 (a.k.a. BRAG1, IQ-ArfGEF, MRX1, MRX18, MRX78)
  • Tag / Fusion Protein
    • His10-TEV (N terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Use BL21 (DE3) Star for expression. PDB structure, 6FAE, is a complex of (ARF1A-c001 & IQSEC2A-c003).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    IQSEC2 (IQSEC2A-c001) was a gift from Nicola Burgess-Brown (Addgene plasmid # 110259 ; http://n2t.net/addgene:110259 ; RRID:Addgene_110259)
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