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Addgene

WNK3 (WNK3B-c016)
(Plasmid #110252)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 110252 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pNIC28-Bsa4
  • Backbone manufacturer
    Opher Gileadi (Addgene plasmid # 26103)
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    WNK3
  • Alt name
    WNK lysine deficient protein kinase 3
  • Species
    H. sapiens (human)
  • Entrez Gene
    WNK3 (a.k.a. PRKWNK3)
  • Promoter T7 - lacO
  • Tag / Fusion Protein
    • His-TEV (N terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Use BL21(DE3)-R3-pRARE2 for expression. 5O26: https://www.thesgc.org/structures/5O26.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    WNK3 (WNK3B-c016) was a gift from Nicola Burgess-Brown (Addgene plasmid # 110252 ; http://n2t.net/addgene:110252 ; RRID:Addgene_110252)
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