KLHL3 (KLHL3A-c003)
(Plasmid
#110251)
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PurposeBacterial expression for structure determination; may not be full ORF
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 110251 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepNIC28-Bsa4
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Backbone manufacturerOpher Gileadi (Addgene plasmid # 26103)
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameKLHL3
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Alt namekelch like family member 3
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SpeciesH. sapiens (human)
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Entrez GeneKLHL3 (a.k.a. PHA2D)
- Promoter T7 - lacO
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Tag
/ Fusion Protein
- His-TEV (N terminal on insert)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer T7
- 3′ sequencing primer T7-term (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Use BL21(DE3)-R3-pRARE2 for expression. 4CH9: https://www.thesgc.org/structures/4CH9.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
KLHL3 (KLHL3A-c003) was a gift from Nicola Burgess-Brown (Addgene plasmid # 110251 ; http://n2t.net/addgene:110251 ; RRID:Addgene_110251)
