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Addgene

pBABE puro human IRS-1
(Plasmid #11025)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 11025 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBABE puro
  • Backbone manufacturer
    available at Addgene
  • Backbone size w/o insert (bp) 5169
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    IRS-1
  • Alt name
    Insulin Receptor Substrate 1
  • Alt name
    IRS1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3750
  • GenBank ID
    NM_005544
  • Entrez Gene
    IRS1 (a.k.a. HIRS-1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer pBABE5'
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBABE puro human IRS-1 was a gift from Ronald Kahn (Addgene plasmid # 11025 ; http://n2t.net/addgene:11025 ; RRID:Addgene_11025)

  • For your References section:

    Differential signaling by insulin receptor substrate 1 (IRS-1) and IRS-2 in IRS-1-deficient cells. Bruning JC, Winnay J, Cheatham B, Kahn CR. Mol Cell Biol. 1997 Mar . 17(3):1513-21. PubMed 9032279
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