E. coli K-12 MG1655_fLP2(yieN-trkD)
(Bacterial strain
#110242)
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PurposeE. coli K-12 MG1655 with Landing Pad engineered into the yieN-trkD intergenic region. The Landing Pad consists of floxed mCherry,Chlor that are cotranscribed.
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Bacterial Strain | 110242 | Bacteria in agar stab | 1 | $85 | |
Backbone
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Vector backbonen/a
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)E. coli K-12 MG1655_fLP2(yieN-trkD)
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameE. coli K-12 MG1655_fLP2(yieN-trkD)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Genotype: F- lambda- ilvG- rfb-50 rph-1 lox71 mCherry,ChlorR loxm2/66
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
E. coli K-12 MG1655_fLP2(yieN-trkD) was a gift from Sriram Kosuri (Addgene plasmid # 110242) -
For your References section:
Systematic Dissection of Sequence Elements Controlling sigma70 Promoters Using a Genomically-Encoded Multiplexed Reporter Assay in E. coli. Urtecho G, Tripp AD, Insigne K, Kim H, Kosuri S. Biochemistry. 2018 Feb 1. doi: 10.1021/acs.biochem.7b01069. 10.1021/acs.biochem.7b01069 PubMed 29388765
Map uploaded by the depositor.
