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Addgene

pAW1
(Plasmid #110220)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 110220 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBluescript
  • Vector type
    Cre/Lox

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ura4+
  • Species
    S. pombe (fission yeast)
  • Mutation
    See depositor comments below.
  • Entrez Gene
    ura4 (a.k.a. SPCC330.05c)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Addgene NGS results detected a P127S variant in Ura4. This variant does not affect plasmid function as described in the associated publication.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAW1 was a gift from Tony Carr (Addgene plasmid # 110220 ; http://n2t.net/addgene:110220 ; RRID:Addgene_110220)

  • For your References section:

    Gene tagging and gene replacement using recombinase-mediated cassette exchange in Schizosaccharomyces pombe. Watson AT1, Garcia V, Bone N, Carr AM, Armstrong J.. Gene. 2008 Jan 15;407(1-2):63-74 10.1016/j.gene.2007.09.024 PubMed 18054176
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