Skip to main content
Addgene

pOBCol3.6-GFPtpz
(Plasmid #110211)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 110211 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pBC SK+
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 3400
  • Total vector size (bp) 9600
  • Modifications to backbone
    None
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    eGFPtopaz
  • Alt name
    Topaz
  • Alt name
    YFP
  • Species
    A. victoria
  • Insert Size (bp)
    720
  • Mutation
    None
  • Promoter 3.6 kb rat type 1 collagen + first intron
  • Tag / Fusion Protein
    • None

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Sac2 (not destroyed)
  • 3′ cloning site Sal1 (not destroyed)
  • 5′ sequencing primer T3
  • 3′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

GFPtopaz bought from Packard.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pOBCol3.6-GFPtpz was a gift from David Rowe (Addgene plasmid # 110211 ; http://n2t.net/addgene:110211 ; RRID:Addgene_110211)

  • For your References section:

    Use of type I collagen green fluorescent protein transgenes to identify subpopulations of cells at different stages of the osteoblast lineage. Kalajzic I, Kalajzic Z, Kaliterna M, Gronowicz G, Clark SH, Lichtler AC, Rowe D. J Bone Miner Res. 2002 Jan;17(1):15-25. doi: 10.1359/jbmr.2002.17.1.15. 10.1359/jbmr.2002.17.1.15 PubMed 11771662
Related items:
Commonly requested with: