pOBCol2.3-GFPemd
(Plasmid #110210)

• Purpose: Labels cells in the late osteoblast lineage.
• Depositing Lab: David Rowe
• Publication: Kalajzic et al J Bone Miner Res. 2002 Jan;17(1):15-25. doi: 10.1359/jbmr.2002.17.1.15.

Backbone

• Vector backbone: pUC18
• Backbone manufacturer: Unknown
• Backbone size w/o insert (bp): 2700
• Total vector size (bp): 7500
• Modifications to backbone: Hind3 and EcoR1 sites in MCS converted to Sal1 sites with adapters.
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: eGFPemerald
• Alt name: Emerald
• Species: A. victoria
• Insert Size (bp): 720
• Promoter: 2.3 kb rat type 1 collagen + first intron
• Tag / Fusion Protein:
  • None

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Hind3 (destroyed during cloning)
• 3′ cloning site: EcoR1 (destroyed during cloning)
• 5′ sequencing primer: M13reverse
• 3′ sequencing primer: M13forward

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

GFPemerald purchased from Packard.