hOC-GFPtpz
(Plasmid #110207)

• Purpose: Expresses GFP in mature bone cells.
• Depositing Lab: David Rowe
• Publication: Bilic-Curcic et al Genesis. 2005 Oct;43(2):87-98. doi: 10.1002/gene.20156.

Backbone

• Vector backbone: pUC18
• Backbone manufacturer: Unknown
• Backbone size w/o insert (bp): 2700
• Modifications to backbone: None
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: eGFPtopaz
• Species: Aequorea victoria
• Insert Size (bp): 720
• GenBank ID:
• Promoter: Human osteocalcin (BGLAP)
• Tag / Fusion Protein:
  • None

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Hind3 (unknown if destroyed)
• 3′ cloning site: Hind3 (unknown if destroyed)
• 5′ sequencing primer: M13forward
• 3′ sequencing primer: M13reverse

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Received osteocalcin portion of construct from Dr. Edith Gardiner, who was associated with the Garvan Institute in Australia at the time. GFPtopaz was purchased from Packard.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry