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Addgene

pTK-ENH_BsmB1-MCS-Avi
(Plasmid #110205)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 110205 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTK
  • Backbone size w/o insert (bp) 4263
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ENH_BsmB1-MCS-Avi
  • Species
    Synthetic
  • Tag / Fusion Protein
    • Avi_tag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Contains BsmBI LacZ cassete for enhancer of choice cloning by Golden gate. Protein of interest can be inserted at the NcoI and SnaBI restriction sites.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTK-ENH_BsmB1-MCS-Avi was a gift from Tatjana Sauka-Spengler (Addgene plasmid # 110205 ; http://n2t.net/addgene:110205 ; RRID:Addgene_110205)

  • For your References section:

    Tissue-Specific In Vivo Biotin Chromatin Immunoprecipitation with Sequencing in Zebrafish and Chicken. Lukoseviciute M, Ling ITC, Senanayake U, Candido-Ferreira I, Taylor G, Williams RM, Sauka-Spengler T. STAR Protoc. 2020 Jul 31;1(2):100066. doi: 10.1016/j.xpro.2020.100066. eCollection 2020 Sep 18. 10.1016/j.xpro.2020.100066 PubMed 33111104
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