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pBABE hygro MEN1 P12L
(Plasmid #11020)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 11020 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBABE hygro
  • Backbone manufacturer
    available at Addgene
  • Backbone size w/o insert (bp) 5558
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MEN1 P12L
  • Alt name
    multiple endocrine neoplasia I
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2437
  • Mutation
    P12L
  • Entrez Gene
    MEN1 (a.k.a. MEAI, SCG2)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer pBABE5'
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Contains 5' and 3' untranslated regions. Author has deposited the sequence of the insert. Click on "sequence" to view.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBABE hygro MEN1 P12L was a gift from Matthew Meyerson (Addgene plasmid # 11020 ; http://n2t.net/addgene:11020 ; RRID:Addgene_11020)

  • For your References section:

    Menin associates with a trithorax family histone methyltransferase complex and with the hoxc8 locus. Hughes CM, Rozenblatt-Rosen O, Milne TA, Copeland TD, Levine SS, Lee JC, Hayes DN, Shanmugam KS, Bhattacharjee A, Biondi CA, Kay GF, Hayward NK, Hess JL, Meyerson M. Mol Cell. 2004 Feb 27. 13(4):587-97. 10.1016/S1097-2765(04)00081-4 PubMed 14992727
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