pGAL - FFL[SEL]
(Plasmid
#1102)
-
Depositing Lab
-
Publication
-
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 1102 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepRS316 (pBluescript, Ura3)
- Backbone size w/o insert (bp) 4400
-
Vector typeYeast Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberLow Copy
Gene/Insert
-
Gene/Insert namefirefly luciferase
-
Speciesfirefly
-
Insert Size (bp)1800
-
Mutationn/a
-
GenBank IDM15077
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site Kpn1 (not destroyed)
- 5′ sequencing primer n/a
- 3′ sequencing primer M13R (Common Sequencing Primers)
Resource Information
-
Article Citing this Plasmid
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
induction by shifting to galactose media; peroxisomal targeting signal mutated (enzyme localized in cytoplasm)
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pGAL - FFL[SEL] was a gift from Susan Lindquist (Addgene plasmid # 1102 ; http://n2t.net/addgene:1102 ; RRID:Addgene_1102) -
For your References section:
Multiple effects of trehalose on protein folding in vitro and in vivo. Singer MA, Lindquist S. Mol Cell 1998 Apr;1(5):639-48. 10.1016/S1097-2765(00)80064-7 PubMed 9660948