hCCR5-LSSmOrange
(Plasmid
#110194)
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PurposeEncodes for human CCR5 coding sequence tagged with the LSS-mOrange FP
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 110194 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneLSSmOrange-N1
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namehCCR5
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SpeciesH. sapiens (human)
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Entrez GeneCCR5 (a.k.a. CC-CKR-5, CCCKR5, CCR-5, CD195, CKR-5, CKR5, CMKBR5, IDDM22)
- Promoter CMV
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Tag
/ Fusion Protein
- LSSmOrange (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
hCCR5-LSSmOrange was a gift from Sergi Padilla Parra (Addgene plasmid # 110194 ; http://n2t.net/addgene:110194 ; RRID:Addgene_110194) -
For your References section:
Multicolor lifetime imaging and its application to HIV-1 uptake. Starling T, Carlon-Andres I, Iliopoulou M, Kraemer B, Loidolt-Krueger M, Williamson DJ, Padilla-Parra S. Nat Commun. 2023 Aug 17;14(1):4994. doi: 10.1038/s41467-023-40731-x. 10.1038/s41467-023-40731-x PubMed 37591879