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Addgene

hCD4-mOrange
(Plasmid #110192)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 110192 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    mOrange-N1
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    hCD4
  • Species
    H. sapiens (human)
  • Entrez Gene
    CD4 (a.k.a. CD4mut, IMD79, Leu-3, OKT4D, T4)
  • Promoter CMV
  • Tag / Fusion Protein
    • mOrange (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    hCD4-mOrange was a gift from Sergi Padilla Parra (Addgene plasmid # 110192 ; http://n2t.net/addgene:110192 ; RRID:Addgene_110192)

  • For your References section:

    A dynamic three-step mechanism drives the HIV-1 pre-fusion reaction. Iliopoulou M, Nolan R, Alvarez L, Watanabe Y, Coomer CA, Jakobsdottir GM, Bowden TA, Padilla-Parra S. Nat Struct Mol Biol. 2018 Sep;25(9):814-822. doi: 10.1038/s41594-018-0113-x. Epub 2018 Aug 27. 10.1038/s41594-018-0113-x PubMed 30150645
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