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Addgene

pEGFP-PTEN-deltaPDZ
(Plasmid #110182)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 110182 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-C1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4731
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    PTEN
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    1200
  • Mutation
    Deletion of last 4 amino acids
  • Entrez Gene
    Pten (a.k.a. MMAC1, Mmac, TEP1)
  • Promoter CMV
  • Tag / Fusion Protein
    • EGFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site ApaI (not destroyed)
  • 5′ sequencing primer unknown
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEGFP-PTEN-deltaPDZ was a gift from Jose Esteban (Addgene plasmid # 110182 ; http://n2t.net/addgene:110182 ; RRID:Addgene_110182)

  • For your References section:

    PTEN is recruited to the postsynaptic terminal for NMDA receptor-dependent long-term depression. Jurado S, Benoist M, Lario A, Knafo S, Petrok CN, Esteban JA. EMBO J. 2010 Aug 18;29(16):2827-40. doi: 10.1038/emboj.2010.160. Epub 2010 Jul 13. 10.1038/emboj.2010.160 PubMed 20628354
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