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pEGFP-MyosinVa-Ctail
(Plasmid #110169)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 110169 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-C1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4731
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Myosin Va
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1239
  • Mutation
    amino acids 1444-1853
  • Entrez Gene
    Myo5a (a.k.a. 9630007J19Rik, Dbv, MVa, Myo5, MyoVA, Sev-1, d, d-120J, flail, flr)
  • Promoter CMV
  • Tags / Fusion Proteins
    • EGFP (N terminal on backbone)
    • Myc (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer unknown
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEGFP-MyosinVa-Ctail was a gift from Jose Esteban (Addgene plasmid # 110169 ; http://n2t.net/addgene:110169 ; RRID:Addgene_110169)

  • For your References section:

    Motor protein-dependent transport of AMPA receptors into spines during long-term potentiation. Correia SS, Bassani S, Brown TC, Lise MF, Backos DS, El-Husseini A, Passafaro M, Esteban JA. Nat Neurosci. 2008 Apr;11(4):457-66. doi: 10.1038/nn2063. Epub 2008 Mar 2. 10.1038/nn2063 PubMed 18311135
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