pX458_Ruby
(Plasmid #110164)

• Purpose: Cas9 from S. pyogenes with Ruby2, and cloning backbone for sgRNA
• Depositing Lab: Philip Hublitz
• Publication: Hertzog et al Eur J Immunol. 2018 Mar 23. doi: 10.1002/eji.201847483.

Backbone

• Vector backbone: PX458
• Backbone manufacturer: Feng Zhang Addgene #48138
• Total vector size (bp): 9277
• Vector type: Mammalian Expression, CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: hSpCas9
• Alt name: SpCas9-2A-Ruby2
• Alt name: Cas9
• Insert Size (bp): 5400
• Tags / Fusion Proteins:
  • 3xFLAG (N terminal on backbone)
  • Ruby2 (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Unknown (unknown if destroyed)
• 3′ cloning site: Unknown (unknown if destroyed)
• 5′ sequencing primer: CCAAAGAGGTGCTGGACG

Resource Information

• Supplemental Documents:
  • pX458_Ruby.gb
• Articles Citing this Plasmid:
  • 5 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry