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pMT2-HA-RAPGEF2(S1244A/S1248A)
(Plasmid #110163)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 110163 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMT2
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    RAPGEF2 (Rap guanine nucleotide exchange factor 2
  • Alt name
    PDZGEF1
  • Species
    H. sapiens (human)
  • Mutation
    S1244A/S1248A
  • Entrez Gene
    RAPGEF2 (a.k.a. CNrasGEF, NRAPGEP, PDZ-GEF1, PDZGEF1, RA-GEF, RA-GEF-1, RAGEF, Rap-GEP, nRap GEP)
  • Promoter CMV
  • Tag / Fusion Protein
    • HA (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (unknown if destroyed)
  • 3′ cloning site XhoI (unknown if destroyed)
  • 5′ sequencing primer unknown
  • 3′ sequencing primer unknown
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMT2-HA-RAPGEF2(S1244A/S1248A) was a gift from Daniele Guardavaccaro (Addgene plasmid # 110163 ; http://n2t.net/addgene:110163 ; RRID:Addgene_110163)

  • For your References section:

    Control of epithelial cell migration and invasion by the IKKbeta- and CK1alpha-mediated degradation of RAPGEF2. Magliozzi R, Low TY, Weijts BG, Cheng T, Spanjaard E, Mohammed S, van Veen A, Ovaa H, de Rooij J, Zwartkruis FJ, Bos JL, de Bruin A, Heck AJ, Guardavaccaro D. Dev Cell. 2013 Dec 9;27(5):574-85. doi: 10.1016/j.devcel.2013.10.023. Epub 2013 Nov 27. 10.1016/j.devcel.2013.10.023 PubMed 24290981
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