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pcDNA3-HA-eEF2K(S441A/S445A)
(Plasmid #110161)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 110161 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    eEF2K (eukaryotic elongation factor 2 kinase)
  • Alt name
    eEF-2K
  • Species
    H. sapiens (human)
  • Mutation
    S441A/S445A
  • Entrez Gene
    EEF2K (a.k.a. CaMKIII, HSU93850, eEF-2K)
  • Promoter CMV
  • Tag / Fusion Protein
    • HA (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (unknown if destroyed)
  • 3′ cloning site EcoRI (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-HA-eEF2K(S441A/S445A) was a gift from Daniele Guardavaccaro (Addgene plasmid # 110161 ; http://n2t.net/addgene:110161 ; RRID:Addgene_110161)

  • For your References section:

    Coupled activation and degradation of eEF2K regulates protein synthesis in response to genotoxic stress. Kruiswijk F, Yuniati L, Magliozzi R, Low TY, Lim R, Bolder R, Mohammed S, Proud CG, Heck AJ, Pagano M, Guardavaccaro D. Sci Signal. 2012 Jun 5;5(227):ra40. doi: 10.1126/scisignal.2002718. 10.1126/scisignal.2002718 PubMed 22669845
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