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Addgene

pcDNA3-HA-TFAP4(E135A/S139A)
(Plasmid #110157)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 110157 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TFAP4 (transcription factor AP-4)
  • Alt name
    AP-4
  • Alt name
    bHLHc41
  • Species
    H. sapiens (human)
  • Mutation
    E135A/S139A
  • Entrez Gene
    TFAP4 (a.k.a. AP-4, bHLHc41)
  • Promoter CMV
  • Tag / Fusion Protein
    • HA (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (unknown if destroyed)
  • 3′ cloning site NotI (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-HA-TFAP4(E135A/S139A) was a gift from Daniele Guardavaccaro (Addgene plasmid # 110157 ; http://n2t.net/addgene:110157 ; RRID:Addgene_110157)

  • For your References section:

    Proteasome-dependent degradation of transcription factor activating enhancer-binding protein 4 (TFAP4) controls mitotic division. D'Annibale S, Kim J, Magliozzi R, Low TY, Mohammed S, Heck AJ, Guardavaccaro D. J Biol Chem. 2014 Mar 14;289(11):7730-7. doi: 10.1074/jbc.M114.549535. Epub 2014 Feb 5. 10.1074/jbc.M114.549535 PubMed 24500709
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