pcDNA3-HA-BHLHE40(S243A/E248A)
(Plasmid
#110155)
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PurposeExpression of human BHLHE40 (S243A/E248A) in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 110155 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameBHLHE40 (basic helix-loop-helix family member e40)
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SpeciesH. sapiens (human)
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MutationS243A/E248A
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Entrez GeneBHLHE40 (a.k.a. BHLHB2, Clast5, DEC1, HLHB2, SHARP-2, SHARP2, STRA13, Stra14)
- Promoter CMV
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Tag
/ Fusion Protein
- HA (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (unknown if destroyed)
- 3′ cloning site NotI (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3-HA-BHLHE40(S243A/E248A) was a gift from Daniele Guardavaccaro (Addgene plasmid # 110155 ; http://n2t.net/addgene:110155 ; RRID:Addgene_110155) -
For your References section:
USP17- and SCFbetaTrCP--regulated degradation of DEC1 controls the DNA damage response. Kim J, D'Annibale S, Magliozzi R, Low TY, Jansen P, Shaltiel IA, Mohammed S, Heck AJ, Medema RH, Guardavaccaro D. Mol Cell Biol. 2014 Nov 15;34(22):4177-85. doi: 10.1128/MCB.00530-14. Epub 2014 Sep 8. 10.1128/MCB.00530-14 PubMed 25202122
