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Addgene

pSKB3-SaGGR-I206F-L377H (JBEI-7202)
(Plasmid #110152)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 110152 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSKB3
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH10B
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GGR
  • Alt name
    geranylgeranyl reductase
  • Species
    Sulfolobus acidocaldarius
  • Mutation
    SaGGR-I206F-L377H mutant gene
  • Promoter T7
  • Tag / Fusion Protein
    • TEV-cleavable His-tag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer unknown
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSKB3-SaGGR-I206F-L377H (JBEI-7202) was a gift from Jay Keasling (Addgene plasmid # 110152 ; http://n2t.net/addgene:110152 ; RRID:Addgene_110152)

  • For your References section:

    Constructing tailored isoprenoid products by structure-guided modification of geranylgeranyl reductase. Kung Y, McAndrew RP, Xie X, Liu CC, Pereira JH, Adams PD, Keasling JD. Structure. 2014 Jul 8;22(7):1028-36. doi: 10.1016/j.str.2014.05.007. Epub 2014 Jun 19. 10.1016/j.str.2014.05.007 PubMed 24954619
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