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pTKan-p35S::Csy4-pC4H::cogRFP (C90, JBEI-15908)
(Plasmid #110143)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 110143 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTKan-GW-R1R2
  • Vector type
    Bacterial Expression, Plant Expression ; binary vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Csy4
  • Species
    A. thaliana (mustard weed)
  • Entrez Gene
    ATCS (a.k.a. AT2G44350, CITRATE SYNTHASE 4, CSY4, F4I1.16)
  • Tag / Fusion Protein
    • cogDsRed (C terminal on insert)

Cloning Information

Resource Information

  • Supplemental Documents

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTKan-p35S::Csy4-pC4H::cogRFP (C90, JBEI-15908) was a gift from Dominique Loque (Addgene plasmid # 110143 ; http://n2t.net/addgene:110143 ; RRID:Addgene_110143)

  • For your References section:

    Endoribonuclease-Based Two-Component Repressor Systems for Tight Gene Expression Control in Plants. Liang Y, Richardson S, Yan J, Benites VT, Cheng-Yue C, Tran T, Mortimer J, Mukhopadhyay A, Keasling JD, Scheller HV, Loque D. ACS Synth Biol. 2017 May 19;6(5):806-816. doi: 10.1021/acssynbio.6b00295. Epub 2017 Feb 8. 10.1021/acssynbio.6b00295 PubMed 28094975
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