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Addgene

pCZ829
(Plasmid #110137)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 110137 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCFJ210
  • Backbone size w/o insert (bp) 9200
  • Total vector size (bp) 11103
  • Vector type
    Worm Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    mito-GCaMP5
  • Species
    Synthetic
  • Promoter Pcol-19
  • Tag / Fusion Protein
    • mito::GCaMP5

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer GGTACCAGAGCTCACCTAGG
  • 3′ sequencing primer GACTCACTAGTGGGCAGATC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCZ829 was a gift from Andrew Chisholm (Addgene plasmid # 110137 ; http://n2t.net/addgene:110137 ; RRID:Addgene_110137)

  • For your References section:

    C. elegans epidermal wounding induces a mitochondrial ROS burst that promotes wound repair. Xu S, Chisholm AD. Dev Cell. 2014 Oct 13;31(1):48-60. doi: 10.1016/j.devcel.2014.08.002. 10.1016/j.devcel.2014.08.002 PubMed 25313960
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