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Addgene

pCZGY2162
(Plasmid #110136)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 110136 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Gateway Final LR Clone
  • Backbone size w/o insert (bp) 2900
  • Total vector size (bp) 5966
  • Vector type
    Worm Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    mito::GCaMP3
  • Alt name
    human Mitochondrial Targeting cytochrome c oxidase
  • Species
    H. sapiens (human), Synthetic
  • Insert Size (bp)
    1452
  • Promoter Pcol-19
  • Tag / Fusion Protein
    • mito::GCaMP3

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ sequencing primer tgtaaaacgacggccagt
  • 3′ sequencing primer caggaaacagctatgaccatg
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCZGY2162 was a gift from Andrew Chisholm (Addgene plasmid # 110136 ; http://n2t.net/addgene:110136 ; RRID:Addgene_110136)

  • For your References section:

    C. elegans epidermal wounding induces a mitochondrial ROS burst that promotes wound repair. Xu S, Chisholm AD. Dev Cell. 2014 Oct 13;31(1):48-60. doi: 10.1016/j.devcel.2014.08.002. 10.1016/j.devcel.2014.08.002 PubMed 25313960
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