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Addgene

FGFR2 L617V 3xFlag
(Plasmid #110110)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 110110 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    p3xFlag-CMV-13
  • Backbone manufacturer
    Sigma
  • Backbone size w/o insert (bp) 6300
  • Total vector size (bp) 8763
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    fibroblast growth factor receptor 2
  • Alt name
    FGFR2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2463
  • Mutation
    mutated Leucine 617 to Valine (L617V)
  • GenBank ID
    NM_000141.4
  • Entrez Gene
    FGFR2 (a.k.a. BBDS, BEK, BFR-1, CD332, CEK3, CFD1, ECT1, JWS, K-SAM, KGFR, TK14, TK25)
  • Promoter CMV
  • Tag / Fusion Protein
    • Flag (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Xba (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer hGH-PA-R
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FGFR2 L617V 3xFlag was a gift from Martin Fernandez-Zapico (Addgene plasmid # 110110 ; http://n2t.net/addgene:110110 ; RRID:Addgene_110110)
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