EGFR WT
(Plasmid #11011)

• Purpose: Retroviral construct for expressing human EGFR in human cells
• Depositing Lab: Matthew Meyerson
• Publication: Greulich et al PLoS Med. 2005 Oct 4. 2(11):e313.

Backbone

• Vector backbone: pBABE puro
• Backbone manufacturer: available at Addgene
• Backbone size w/o insert (bp): 5169
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: EGFR
• Alt name: epidermal growth factor receptor
• Alt name: ERBB
• Species: H. sapiens (human)
• Insert Size (bp): 3639
• Entrez Gene: EGFR (a.k.a. ERBB, ERBB1, ERRP, HER1, NISBD2, NNCIS, PIG61, mENA)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SnaBI (destroyed during cloning)
• 3′ cloning site: SnaBI (destroyed during cloning)
• 5′ sequencing primer: pBABE5'

Resource Information

• Addgene Notes:
  • Addgene Diagnostic Digest
• Articles Citing this Plasmid:
  • 64 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Author has deposited the sequence of the insert to Addgene. Click on "sequence" to view. EGFR was amplified with the primers 5'-GATGATATCATGCGACCCTCCGGGAC-3' and 5'-ATCGATATCTCATGCTCCAATAAATTC-3', digested with EcoRV, and inserted into the SnaB1 site of pBabe-Puro.