pFLAG_attP
(Plasmid #110095)

• Purpose: (Empty Backbone) Vector for low-level constitutive expression in mycobacteria from the Tet promoter. Lacks the Tet repressor. Contains attP for chromosomal integration. Lacks the integrase and thus remains stably integrated in the absence of antibiotic selection. Contains a C-terminal 3xFLAG to allow detection of expression levels by Western blotting.
• Depositing Lab: Markus Seeger
• Publication: Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.1038/s41598-018-27687-5.

Backbone

• Vector backbone: pFLAG
• Vector type: Bacterial Expression
• Tag / Fusion Protein:
  • 3xFLAG (C terminal on backbone)

Growth in Bacteria

• Bacterial Resistance(s): Apramycin and Chloramphenicol
• Growth Temperature: 37°C
• Growth Strain(s): DB3.1
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: None
• Tag / Fusion Protein:
  • 3xFLAG (C terminal on backbone)

Resource Information

• Supplemental Documents:
  • pFLAG_attP.gbk
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry