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Addgene

pYC6H-TRP1URA3
(Plasmid #11009)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 11009 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pYC6-CT/HIS3 hybrid
  • Backbone size w/o insert (bp) 3786
  • Vector type
    Bacterial Expression, Yeast Expression
  • Selectable markers
    HIS3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Pgal1TRP1URA3
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    2240
  • Mutation
    ORF-TRP1 fused to ORF-URA without stop codon
  • Tags / Fusion Proteins
    • his6 (C terminal on backbone)
    • V5 (C terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that Addgene's sequencing results identified mutations A160S and K173R in the URA3 sequence. These mutations were not intentional and the depositing scientist states that the plasmid was tested for complementation of URA and TRP phenotypes and for production of a single transcript.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pYC6H-TRP1URA3 was a gift from Francisco Malagon (Addgene plasmid # 11009 ; http://n2t.net/addgene:11009 ; RRID:Addgene_11009)
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