pKO (Hyg)
(Plasmid
#110087)
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Purpose(Empty Backbone) Suicide vector for generation of gene deletions in mycobacteria. Contains the two genes sacB and galK for negative selection.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 110087 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepKO
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Vector typeBacterial Knockout Generation
Growth in Bacteria
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Bacterial Resistance(s)Hygromycin, 200 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pKO (Hyg) was a gift from Markus Seeger (Addgene plasmid # 110087 ; http://n2t.net/addgene:110087 ; RRID:Addgene_110087) -
For your References section:
A uniform cloning platform for mycobacterial genetics and protein production. Arnold FM, Hohl M, Remm S, Koliwer-Brandl H, Adenau S, Chusri S, Sander P, Hilbi H, Seeger MA. Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.1038/s41598-018-27687-5. 10.1038/s41598-018-27687-5 PubMed 29934571
