pACEC3GH (Hyg)
(Plasmid
#110084)
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Purpose(Empty Backbone) Replicative, acetamide-based expression vector containing a C-terminal 3C cleavage site and a GPF-fusion with a His10-tag. Suitable for high-level expression levels amenable for large scale purification of proteins.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 110084 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepACEC3GH
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Vector typeBacterial Expression
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Tag
/ Fusion Protein
- 3C cleavage site - GFP - His10 Tag (C terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Hygromycin, 200 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
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Tag
/ Fusion Protein
- 3C cleavage site - GFP - His10 Tag (C terminal on backbone)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pACEC3GH (Hyg) was a gift from Markus Seeger (Addgene plasmid # 110084 ; http://n2t.net/addgene:110084 ; RRID:Addgene_110084) -
For your References section:
A uniform cloning platform for mycobacterial genetics and protein production. Arnold FM, Hohl M, Remm S, Koliwer-Brandl H, Adenau S, Chusri S, Sander P, Hilbi H, Seeger MA. Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.1038/s41598-018-27687-5. 10.1038/s41598-018-27687-5 PubMed 29934571
