pMINTNH3
(Plasmid
#110074)
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Purpose(Empty Backbone) Integrative vector for expression in mycobacteria based on the Tet promoter. Contains an N-terminal His10 Tag followed by a 3C cleavage site
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 110074 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepMINTNH3
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Vector typeBacterial Expression
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Tag
/ Fusion Protein
- His10 Tag - 3C cleavage site (N terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Kanamycin, 25 & 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
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Tag
/ Fusion Protein
- His10 Tag - 3C cleavage site (N terminal on backbone)
Resource Information
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Supplemental Documents
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Addgene Notes
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMINTNH3 was a gift from Markus Seeger (Addgene plasmid # 110074 ; http://n2t.net/addgene:110074 ; RRID:Addgene_110074) -
For your References section:
A uniform cloning platform for mycobacterial genetics and protein production. Arnold FM, Hohl M, Remm S, Koliwer-Brandl H, Adenau S, Chusri S, Sander P, Hilbi H, Seeger MA. Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.1038/s41598-018-27687-5. 10.1038/s41598-018-27687-5 PubMed 29934571
