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Pet28a His6-HSPB7 (zebrafish) C49S/C117S
(Plasmid #110073)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 110073 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pet28a
  • Backbone size w/o insert (bp) 5186
  • Total vector size (bp) 5741
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    His6-HSPB7
  • Alt name
    Heat shock protein family B (small) member 7
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
    555
  • Mutation
    Cysteine 49 to Serine, Cysteine 117 to Serine
  • Entrez Gene
    hspb7 (a.k.a. zgc:103426)
  • Promoter T7
  • Tag / Fusion Protein
    • His6 (N terminal on insert)

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer TAA TAC GAC TCA CTA TAG GG
  • 3′ sequencing primer gct agt tat tgc tca gcg g
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Thrombin cleavage site between N-terminal His tag and protein

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Pet28a His6-HSPB7 (zebrafish) C49S/C117S was a gift from Yimon Aye (Addgene plasmid # 110073 ; http://n2t.net/addgene:110073 ; RRID:Addgene_110073)

  • For your References section:

    Cardiovascular Small Heat Shock Protein HSPB7 Is a Kinetically Privileged Reactive Electrophilic Species (RES) Sensor. Surya SL, Long MJC, Urul DA, Zhao Y, Mercer EJ, EIsaid IM, Evans T, Aye Y. ACS Chem Biol. 2018 Feb 8. doi: 10.1021/acschembio.7b00925. 10.1021/acschembio.7b00925 PubMed 29397684
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