pYES2.1-TRP1
(Plasmid
#11007)
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 11007 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepYES2.1/V5-His-TOPO
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5886
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Vector typeBacterial Expression, Yeast Expression
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Selectable markersURA3
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTRP1
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SpeciesS. cerevisiae (budding yeast)
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Insert Size (bp)672
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MutationORF-TRP1 without stop codon
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Entrez GeneTRP1 (a.k.a. YDR007W)
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Tags
/ Fusion Proteins
- his6 (C terminal on backbone)
- V5 (C terminal on backbone)
Cloning Information
- Cloning method TOPO Cloning
- 5′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pYES2.1-TRP1 was a gift from Francisco Malagon (Addgene plasmid # 11007 ; http://n2t.net/addgene:11007 ; RRID:Addgene_11007)