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Halo-Tev-Flag-Ube2v1
(Plasmid #110069)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 110069 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pfn21a
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 3760
  • Total vector size (bp) 5236
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Halo-Tev-Flag-Ube2v1
  • Alt name
    Ubiquitin-conjugating enzyme E2 variant 1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1476
  • Entrez Gene
    UBE2V1 (a.k.a. CIR1, CROC-1, CROC1, UBE2V, UEV-1, UEV1, UEV1A)
  • Promoter T7
  • Tag / Fusion Protein
    • Halo-TEV-Flag (N terminal on insert)

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer GCAAAGCCACCATGGCAGAAATCGGTACTGGCTTTCCATTCGACCCCCATTATGTGGAAG
  • 3′ sequencing primer CCGAGCCCGAATTCGTTTAATTGCTGTAACACTGTCCTTCGGGCGGCTGAGGGAGTTTCA
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Halo-Tev-Flag-Ube2v1 was a gift from Yimon Aye (Addgene plasmid # 110069 ; http://n2t.net/addgene:110069 ; RRID:Addgene_110069)

  • For your References section:

    Ube2V2 Is a Rosetta Stone Bridging Redox and Ubiquitin Codes, Coordinating DNA Damage Responses. Zhao Y, Long MJC, Wang Y, Zhang S, Aye Y. ACS Cent Sci. 2018 Feb 28;4(2):246-259. doi: 10.1021/acscentsci.7b00556. Epub 2018 Jan 17. 10.1021/acscentsci.7b00556 PubMed 29532025
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