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Addgene

pSY091
(Plasmid #110068)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 110068 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET30

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH10B
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    L4
  • Species
    Synthetic
  • Mutation
    AT-swapped mutant

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unkown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer unknown
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSY091 was a gift from Jay Keasling (Addgene plasmid # 110068 ; http://n2t.net/addgene:110068 ; RRID:Addgene_110068)

  • For your References section:

    Comprehensive in Vitro Analysis of Acyltransferase Domain Exchanges in Modular Polyketide Synthases and Its Application for Short-Chain Ketone Production. Yuzawa S, Deng K, Wang G, Baidoo EE, Northen TR, Adams PD, Katz L, Keasling JD. ACS Synth Biol. 2017 Jan 20;6(1):139-147. doi: 10.1021/acssynbio.6b00176. Epub 2016 Sep 6. 10.1021/acssynbio.6b00176 PubMed 27548700
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