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FUW mCherry-GFP-LC3
(Plasmid #110060)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 110060 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    FUW
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Growth instructions
    Plasmid may be prone to recombination. Select small colonies and/or screen multiple colonies.
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    mCherry-GFP-LC3

Cloning Information

  • Cloning method TOPO Cloning
  • 5′ sequencing primer CGCGGATCCGGTCGCCACCATGGTGAGCAAGGGCGAG
  • 3′ sequencing primer CGCGGCGCGCCGCTGGGTCTAGATGCATGC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FUW mCherry-GFP-LC3 was a gift from Anne Brunet (Addgene plasmid # 110060 ; http://n2t.net/addgene:110060 ; RRID:Addgene_110060)

  • For your References section:

    Lysosome activation clears aggregates and enhances quiescent neural stem cell activation during aging. Leeman DS, Hebestreit K, Ruetz T, Webb AE, McKay A, Pollina EA, Dulken BW, Zhao X, Yeo RW, Ho TT, Mahmoudi S, Devarajan K, Passegue E, Rando TA, Frydman J, Brunet A. Science. 2018 Mar 16;359(6381):1277-1283. doi: 10.1126/science.aag3048. Epub 2018 Mar 15. 10.1126/science.aag3048 PubMed 29590078
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