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PurposePer2 transcription fluorescence reporter
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 110058 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneaav2
- Backbone size w/o insert (bp) 4171
- Total vector size (bp) 6454
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Vector typeMammalian Expression, AAV, Cre/Lox
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePer2 promoter and mCherry and Venus
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Alt namePer2 promoter
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SpeciesM. musculus (mouse)
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GenBank ID
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Entrez GenePer2 (a.k.a. mKIAA0347, mPer2)
- Promoter Per2
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Tag
/ Fusion Protein
- NLS-D2 (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer Unknown (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
AAV-P(Per2)-DIO-mCherry-Venus-NLS-D2 was a gift from Eric Erquan Zhang (Addgene plasmid # 110058 ; http://n2t.net/addgene:110058 ; RRID:Addgene_110058) -
For your References section:
Long-term in vivo recording of circadian rhythms in brains of freely moving mice. Mei L, Fan Y, Lv X, Welsh DK, Zhan C, Zhang EE. Proc Natl Acad Sci U S A. 2018 Apr 2. pii: 1717735115. doi: 10.1073/pnas.1717735115. 10.1073/pnas.1717735115 PubMed 29610316