AAV-P(Cry1)-forward-intron336-Venus-NLS-D2
(Plasmid #110054)

• Purpose: Cry1 transcription fluorescence reporter
• Depositing Lab: Eric Erquan Zhang
• Publication: Mei et al Proc Natl Acad Sci U S A. 2018 Apr 2. pii: 1717735115. doi: 10.1073/pnas.1717735115.

Backbone

• Vector backbone: aav2
• Backbone size w/o insert (bp): 4171
• Total vector size (bp): 7688
• Vector type: Mammalian Expression, AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Cry1 promoter and Venus
• Alt name: Cry1 intron336
• Species: M. musculus (mouse)
• GenBank ID:
• Entrez Gene: Cry1 (a.k.a. Phll1)
• Promoter: Cry1
• Tag / Fusion Protein:
  • NLS-D2 (C terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: Unknown

Resource Information

• Supplemental Documents:
  • AAV-P(Cry1)-forward-intron336-Venus-NLS-D2.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid was generated based on a plasmid from the laboratory of Hiroki Ueda. Please also include the following citation when using this plasmid: Delay in Feedback Repression by Cryptochrome 1 Is Required for Circadian Clock Function. Ukai-Tadenuma, Maki et al. Cell, Volume 144, Issue 2, 268 – 281.