pHH0103_SPRED3_WH1
(Plasmid
#109899)
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PurposeProtein expression and purification of SPRED3_WH1
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 109899 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepHH0103
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameSPRED3_WH1
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SpeciesH. sapiens (human)
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Entrez GeneSPRED3 (a.k.a. Eve-3, spred-3)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Sfi1 (unknown if destroyed)
- 3′ cloning site Not1 (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHH0103_SPRED3_WH1 was a gift from Sachdev Sidhu (Addgene plasmid # 109899 ; http://n2t.net/addgene:109899 ; RRID:Addgene_109899) -
For your References section:
Large-scale survey and database of high affinity ligands for peptide recognition modules. Teyra J, Kelil A, Jain S, Helmy M, Jajodia R, Hooda Y, Gu J, D'Cruz AA, Nicholson SE, Min J, Sudol M, Kim PM, Bader GD, Sidhu SS. Mol Syst Biol. 2020 Dec;16(12):e9310. doi: 10.15252/msb.20199310. 10.15252/msb.20199310 PubMed 33438817