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Addgene

pL-rpb1-E1103G
(Plasmid #10989)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 10989 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRS415 (with modifications)
  • Backbone size w/o insert (bp) 4967
  • Vector type
    Bacterial Expression, Yeast Expression
  • Selectable markers
    LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    rpb1-E1103G
  • Alt name
    RPB1
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    6349
  • Mutation
    Mutation 3308 A to G in the RPB1 ORF resulting in the residue substitution E1103G
  • Entrez Gene
    RPO21 (a.k.a. YDL140C, RPB1, RPB220, SIT1, SUA8)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI/AvaI (not destroyed)
  • 3′ cloning site ApaI (not destroyed)
  • 5′ sequencing primer M13 reverse
  • 3′ sequencing primer n/a
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pL-rpb1-E1103G was a gift from Jeff Strathern (Addgene plasmid # 10989 ; http://n2t.net/addgene:10989 ; RRID:Addgene_10989)

  • For your References section:

    Mutations in the Saccharomyces cerevisiae RPB1 Gene Conferring Hypersensitivity to 6-Azauracil. Malagon F, Kireeva ML, Shafer BK, Lubkowska L, Kashlev M, Strathern JN. Genetics. 2006 Apr . 172(4):2201-9. 10.1534/genetics.105.052415 PubMed 16510790
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